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胡凯锋研究员

    
 
 
 

      胡凯锋,博士、研究员、博士生导师、毕业于瑞士联邦苏黎世高等理工学院,曾在美国NIH任研究助理,美国威斯康星大学任助理科学家。现任成都中医药大学,中医药创新研究院研究员,课题组长;中国生物物理学会生物磁共振专业委员会委员,中国生物物理学会代谢组学分会理事,中国化学会有机分析专业委员会委员,中国医药生物技术协会药物分析技术分会委员;中共云南省委联系专家,四川省“特聘专家”。

      主要结合核磁共振技术和液质联用技术开展以下领域研究:(1)代谢组学;(2)结构生物学;(3)复杂混合物核磁共振和液质联用技术定性、定量方法开发。(4)中药药效物质基础与作用机制。

      注重理论和技术方法创新,开发了一序列用于生物大分子研究的核磁共振新方法,以及用于代谢组学研究的定性、定量分析方法。拓展新技术、新方法的应用研究,借鉴代谢组学分析策略用于药效物质成分分析,开展疾病相关蛋白质的结构与功能关系研究,发展改良核磁共振方法用于靶向蛋白质的活性分子筛选研究。发表论文近40多篇。

      研究组网页:http://www.hunmrlab.cn/

      主持科技部国家重点研发计划“精准医学研究”重点专项课题(课题负责人),国家自然科学基金面上项目,云南省应用基础研究计划重点项目,以及多项国家及省部级人才项目;

 
 

(一)代谢组学成果

1.Tao Huang, Pengyu Chen, Bin Liu, Xing Li, Xinqiao Lv, Kaifeng Hu*, NPid: an automatic approach to rapid identification of known natural products in the crude extract of crabapple based on 2D 1H-13C heteronuclear correlation spectra of the extract mixture, Analytical Chemistry, 2020, accepted, DOI:

2.Bin Liu#, Yanli Chen#, Xiaofang Ma, Kaifeng Hu*, Site-specific peak intensity ratio (SPIR) from 1D 2H/1H NMR spectra for rapid distinction between natural and synthetic nicotine and detection of possible adulteration, Analytical and Bioanalytical Chemistry, 2019, 411(24), 6427–6434.

3.Xing Li, Huan Luo, Tao Huang, Li Xu, Xiaohuo Shi, Kaifeng Hu*, Statistically correlating NMR spectra and LC-MS data to facilitate the identification of individual metabolite in metabolomics mixtures, Analytical and Bioanalytical Chemistry, 2019, 411(7), 1301-1309

4.Tao Huang, Lingling Yu, Xiaofang Ma, Kaifeng Hu*, An adapted isotope dilution 1H-13C heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose, Analytical and Bioanalytical Chemistry, 2018, 410(26), 6705-6711.

5.Lingling Yu, Chao Wen, Xing Li, Shiqi Fang, Lichuan Yang, Tony Wang, Kaifeng Hu*, Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag, Analytical and Bioanalytical Chemistry, 2018, 410(7), 2011-2018.

6.Chao Wen, Dongshan Wang, Xing Li, Tao Huang, Cheng Huang, Kaifeng Hu*, Targeted isolation and identification of bioactive compounds lowering cholesterol in the crude extracts of crabapples using UPLC-DAD-MS-SPE/NMR based on pharmacology-guided PLS-DA, Journal of Pharmaceutical and Biomedical Analysis, 2018, 150,144-151.

7.Kaifeng Hu, William M. Westler and John L. Markley*, Simultaneous quantification and identification of individual chemicals in metabolite mixtures by two-dimensional extrapolated time-zero 1H-13C HSQC (HSQC0). J. Am. Chem. Soc., 2011, 133(6), 1662-5.

8.Kaifeng Hu*, James J. Ellinger, Roger A. Chylla and John L. Markley, Measurement of absolute concentrations of individual compounds in metabolite mixtures by gradient-selective time-zero 1H-13C HSQC (gsHSQC0) with two concentration references and fast maximum likelihood reconstruction analysis. Analytical Chemistry, 2011, 83(24), 9352-60.

(二)结构生物学成果

9.Xiaofang Ma, Bin Liu, Jiahui Yang*, Kaifeng Hu*, Solution Structure, Dynamics and Function Investigation of Kringle Domain of Human Receptor Tyrosine Kinase-like Orphan Receptor 1, Journal of Biomolecular Structure & Dynamics, 2020, 38(8), 2229-2239.

10.Bin Liu#, Yan Hou#, Xiaozheng Wang, Xiaofang Ma, Shiqi Fang, Tao Huang, Yanli Chen, Zhiqiang Bai, Shuangjun Lin*, Rundong Zhang*, Kaifeng Hu*, Structural basis of the mechanism of β-methyl epimerization by enzyme MarH, Organic & Biomolecular Chemistry, 2019, 17(44), 9605–9614.

11.Mingqiang Rong#, Jiangxin Liu#, Meilin Zhang#, Gan Wang, Gang Zhao, GuoDong Wang, YaPing Zhang, Kaifeng Hu*, Ren Lai*, A sodium channel inhibitor ISTX-I with a novel structure provides a new hint at the evolutionary link between two toxin folds, Scientific Reports, 2016, 6 :29691.

12.Jun Hu#, Kaifeng Hu#, David C. Williams Jr., Michal Komlosh, Mengli Cai and G. Marius Clore*, Solution NMR structures of productive and non-productive complexes between the A and B domains of the cytoplasmic subunit of the mannose transporter of the Escherichia coli phosphotransferase system. J. Biol. Chem., 2008, 283 (16), 11024-11037.

(三)核磁共振理论方法

13.Kaifeng Hu, Beat V?geli and G. Marius Clore*, Spin state selective 13C-detected HNCO with TROSY optimization in all dimensions and double echo-antiecho sensitivity enhancement in both indirect dimensions. J. Am. Chem. Soc., 2007, 129(17), 5484-91.

    
 

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